The SP8 is equipped with water immersion dipping objectives, which are useful to researchers who wish to perform live imaging on whole animals or large tissue preparations, and for electrophysiology experiments. It is also the best choice for imaging non-fluorescent materials via reflection mode.

Objectives

• Water immersion ceramic objectives
• 25X/0.9 N.A. (HCX IRAPO)+ (2.5 mm free working distance)
• 40X/0.8 N.A. (HCX APO L U-V-I)+ (3.3 mm free working distance)
• 63X/0.9 N.A. (HCX APO)+ (2.2 mm free working distance)
• Plan apochromatic oil immersion objectives
• 40X/1.30 N.A. (HC PL APO CS2) (0.24 mm free working distance)
• 63X/1.40 N.A. (HC PL APO CS2) (0.14 mm free working distance)
• 100X/1.40 N. A. (HC PL APO CS2)+ (0.13 mm free working distance)
• Dry objectives
• 20X/ 0.75 N.A. (HC PLAPO) high and dry plan apochromatic (0.62 mm free working distance)
• 10X/0.3 N.A. (HC PL fluotar) (11 mm free working distance)
• (DIC imaging is available with all objectives)

(+If you wish to use any of these objectives, please notify the Core Manager, as they are kept off the microscope when not in use.)

Lasers

• 405 nm continuous wave laser
• 458, 476, 488, 496, and 514 nm (Multi-line argon laser)
• 561 nm (DPSS Yellow laser)
• 594 nm (Orange HeNe laser)
• 633 nm (Red HeNe laser)

Other Features

• Hamamatsu EM-CCD camera system
• Tandem Scanner with Conventional scanner and 12kHz Resonant high speed (capable of 12,000 lines per second!) scanner combination.
• Custom built translational stage (for imaging slides to large rodents)
• Scanning stage also available (for mosaic tile scans)
• Trigger unit box for electrophysiology experiments
• Metal halide lamp for Fura 2 Ca2+ imaging
• 3 Hamamatsu R 9624 PMTs
• 2 ultrasensitive HyD GaAsP detectors (30% quantum efficiency)
• Acousto Optical Beam Splitter
• SP-Prism Based True Spectral Detection
• Transmitted light detector
• Epifluorescence visualization via eyepieces

Applications

• Simultaneous 5-color imaging
• Fast and sensitive Fura-2 Ca2+ imaging
• Photon counting (w/ HyD detectors)
• Time lapse imaging
• 3-dimensional imaging
• Time lapse and 3-D animations
• FRAP
• FRET
• DIC imaging
• Lambda scans
• Materials Science imaging using the reflected light mode

Read protocols for the Leica TCS SP8…